At the University of Gent, on the 24th of November dr. Marcel Schmidt (Enzypep) will give a lecture on enzymatic ligation.
Enzymatic Ligation as a Tool for Assembling Peptides & Conjugates
Peptiligases are protein engineered variants of subtilisin that catalyse the coupling of peptides to peptides and proteins in an irreversible manner enabling high conversion (>90%) to the final product (peptide, conjugate, fusion protein). The reactions are very fast (minutes) and take place in aqueous media at neutral pH with (for peptides) close to equimolar concentrations of reactants. Initially developed to assemble long peptides from fragments, it was recently recognized that these enzymes are capable of coupling peptides to proteins with accessible N-terminal amines. Model peptides (up to 45 amino acids) have been attached to many proteins, including XTEN, human serum albumin, insulin, and thyroglobulin. Selective ligases have been developed that permit chain-selective coupling of peptides to dimeric proteins (e.g. insulin). The technology has some obvious applications for biologics such as site-specific conjugation, fusion proteins with non-standard amino acids, and replacing sortases and native chemical ligation as procedures for coupling polypeptides.
Date and place:
Thursday November 24th 2016 at 14.00h
Auditorium D, S4bis
Department of Organic and Macromolecular Chemistry
Krijgslaan 281, B-9000 Gent
Prof. A. Madder
Dr. Marcel Schmidt